
Vitamin D (25-Hydroxy) near me in Pune







The Vitamin D (25-Hydroxy) test is tailored to measure vitamin D levels in the body and is available at an affordable price in Pune with Tata 1mg labs. Vitamin D is a fat-soluble essential nutrient that helps the body maintain strong bones & teeth and a healthy immune system. It can be obtained from healthy exposure to sunlight and dietary sources.
Your doctor may suggest the Vitamin D (25-Hydroxy) test if they notice health issues associated with low vitamin D levels like an increased risk of fractures, muscle weakness and lethargy, dysfunction of the immune system, and increased risk of chronic diseases like diabetes. This test also monitors the treatment response in people receiving medication for abnormal vitamin D levels.
It is generally recommended to stop taking vitamin D supplements before taking Vitamin D (25-Hydroxy) test, as they may affect the accuracy of the test results. However, you may consume a regular diet before taking the test.


What does Vitamin D (25-Hydroxy) measure?
The Vitamin D (25-Hydroxy) test measures the levels of vitamin D in the body. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. It exists in two forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D2 is present in plants, such as yeast or mushrooms, and is available as a supplement in fortified foods. Vitamin D3 is found in foods like cheese, green vegetables, mushrooms, egg yolks, and fatty fish.
Both forms of vitamin D need to be converted in the liver and kidneys before the body can use them effectively. This test shows the total level of vitamin D (D2 + D3) circulating in the body and guides your doctor in recommending the right treatment or lifestyle changes if needed.





- Vitamin D [Internet]. NIH; 12 Aug. 2022 [Accessed 03 Nov. 2025]. Available from:
- Aparna P, Muthathal S, Nongkynrih B, Gupta SK. Vitamin D deficiency in India. J Family Med Prim Care. 2018 Mar-Apr;7(2):324-330. [Accessed 03 Nov. 2025]. Available from:
- Vitamin D [Internet]. Boston, USA: Harvard T.H. Chan; March 2023 [Accessed 03 Nov. 2025]. Available from:
- Vähävihu K, Ylianttila L, Salmelin R, Lamberg-Allardt C, Viljakainen H, Tuohimaa P, Reunala T, Snellman E. Heliotherapy improves vitamin D balance and atopic dermatitis. Br J Dermatol. 2008 Jun;158(6):1323-8. [03 Nov. 2025]. Available from:
- Devulapalli CS. Physical activity and vitamin D in children: a review of impacts on bone health and fitness. J Pediatr Endocrinol Metab. 2025 Mar 4. [03 Nov. 2025]. Available from:
- Quraishi SA, Camargo CA Jr. Vitamin D in acute stress and critical illness. Curr Opin Clin Nutr Metab Care. 2012 Nov;15(6):625-34. [03 Nov. 2025]. Available from:
- Al-Bashaireh AM, Haddad LG, Weaver M, Chengguo X, Kelly DL, Yoon S. The Effect of Tobacco Smoking on Bone Mass: An Overview of Pathophysiologic Mechanisms. J Osteoporos. 2018 Dec 2;2018:1206235. [03 Nov. 2025]. Available from:
- Sampson HW. Alcohol's harmful effects on bone. Alcohol Health Res World. 1998;22(3):190-4. PMID: 15706795; PMCID: PMC6761900. [03 Nov. 2025]. Available from:
- Pilz S, Zittermann A, Trummer C, Theiler-Schwetz V, Lerchbaum E, Keppel MH, Grübler MR, März W, Pandis M. Vitamin D testing and treatment: a narrative review of current evidence. Endocr Connect. 2019 Feb 1;8(2):R27-R43. [03 Nov. 2025]. Available from:
- Wagner CL, Taylor SN, Johnson DD, Hollis BW. The role of vitamin D in pregnancy and lactation: emerging concepts. Womens Health (Lond). 2012 May;8(3):323-40. [03 Nov. 2025]. Available from:
- Domenici R, Vierucci F. Exclusive Breastfeeding and Vitamin D Supplementation: A Positive Synergistic Effect on Prevention of Childhood Infections? Int J Environ Res Public Health. 2022 Mar 3;19(5):2973.[03 Nov. 2025]. Available from:
- Vranić L, Mikolašević I, Milić S. Vitamin D Deficiency: Consequence or Cause of Obesity? Medicina (Kaunas). 2019 Aug 28;55(9):541. [03 Nov. 2025]. Available from:
- Tardelli VS, Lago MPPD, Silveira DXD, Fidalgo TM. Vitamin D and alcohol: A review of the current literature. Psychiatry Res. 2017 Feb;248:83-86. [Accessed 03 Nov. 2025]. Available from:
- Luo T, Lin Y, Lu J, Lian X, Guo Y, Han L, Guo Y. Effects of vitamin D supplementation during pregnancy on bone health and offspring growth: A systematic review and meta-analysis of randomized controlled trials. PLoS One. 2022 Oct 13;17(10):e0276016. [Accessed 03 Nov. 2025]. Available from:
- Giustina A, Bouillon R, Dawson-Hughes B, Ebeling PR, Lazaretti-Castro M, Lips P, Marcocci C, Bilezikian JP. Vitamin D in the older population: a consensus statement. Endocrine. 2023 Jan;79(1):31-44. [Accessed 03 Nov. 2025]. Available from:
- Nowak J, Hudzik B, Jagielski P, Kulik-Kupka K, Danikiewicz A, Zubelewicz-Szkodzińska B. Lack of Seasonal Variations in Vitamin D Concentrations among Hospitalized Elderly Patients. Int J Environ Res Public Health. 2021 Feb 9;18(4):1676. [Accessed 03 Nov. 2025]. Available from:
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